Single cell type - PPP2R2B - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	PPP2R2B
Gene descriptionⁱ Full gene name according to HGNC.	Protein phosphatase 2 regulatory subunit Bbeta
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s).	Disease related genes Human disease related genes
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	10

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity.	Oligodendrocytes - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Cell type enhanced (Oligodendrocytes, Astrocytes, Oligodendrocyte precursor cells, Late spermatids, Excitatory neurons, Inhibitory neurons)
Tau specificity scoreⁱ Tau specificity score is a numerical indicator of the specificity of the gene expression across cells or tissues. The value ranges from 0 and 1, where 0 indicates identical expression across all cells/tissue types, while 1 indicates expression in a single cell/tissue type.	0.78
Brain cluster specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell cluster types based on snRNA-seq data from brain samples. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. "cell type" refer to one of the 34 cluster names used to summarize the 2.5 million cells included in the brain cell representation.	Cell type enhanced (Committed oligodendrocyte precursor, Oligodendrocyte)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Group enriched (memory CD8 T-cell, gdT-cell, MAIT T-cell, naive CD8 T-cell, T-reg, NK-cell, memory CD4 T-cell)
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); neXtProt protein existence (neXtProt evidence);and a Human Protein Atlas antibody- or RNA based score (HPA evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable.	Evidence at protein level

ASSAYSⁱ

Single cell type

Cell type enrichment

Single nuclei - Brain

Immune cells

Expression clustering

SINGLE CELL TYPESⁱ A summary of normalized single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types RNA single cell type specificity: Cell type enhanced (Oligodendrocytes, Astrocytes, Oligodendrocyte precursor cells, Late spermatids, Excitatory neurons, Inhibitory neurons)

TISSUE CELL TYPEⁱ The tissue cell type section provides a cell type specificity prediction for all protein coding genes. More information on the data available can be found here and a description of the analysis here.
	Core cell typesⁱ The core cell type table displays data for a panel of eight cell types that are found in many tissues. Colored dots indicate that the selected gene has core cell type specificity in the indicated tissue. Grey dots indicate that cell type is present within that tissue, but the selected gene is not predicted to be enriched there. Category Alphabetical Enrichment Tissue specific cell typesⁱ This plot shows the enrichment score of the selected gene in all non-core cell types found within the profiled tissues, allowing comparisons between different cell types within different tissues. An enlarged symbol indicates classification of the gene as cell type enriched in the corresponding cell type.

SINGLE NUCLEI BRAINⁱ

Single nuclei cluster types

RNA brain cluster specificity: Cell type enhanced (Committed oligodendrocyte precursor, Oligodendrocyte)

IMMUNE CELL TYPE EXPRESSION (RNA)ⁱ Blood cell type expression overview shows RNA-seq data from three different sources: Internally generated Human Protein Atlas (HPA) data, as well as data generated by (Monaco et al) and (Schmiedel et al). Color-coding is based on blood cell type lineages, including B-cells, T-cells, NK-cells, monocytes, granulocytes and dendritic cells as well as total PBMC. To access sample data, click on tissue name or bar.
HPA datasetⁱ The resulting transcript expression values calculated as nTPM, resulting from the internal normalization pipeline for 18 immune cell types and total peripheral blood mononuclear cells (PBMC). Lineage Expression Alphabetical Immune cell type specificity: Group enriched (memory CD8 T-cell, gdT-cell, MAIT T-cell, naive CD8 T-cell, T-reg, NK-cell, memory CD4 T-cell)
Monaco datasetⁱ The transcript expression values (nTPM) resulting from the internal normalization pipeline are visualized for 29 blood cell types and total peripheral blood mononuclear cells (PBMC) from Monaco et al. Lineage Expression Alphabetical
Schmiedel datasetⁱ Transcript Per Million (TPM) values are visualized for 15 blood cell types from the Schmiedel et al. dataset. Lineage Expression Alphabetical

EXPRESSION CLUSTERING & CORRELATIONⁱ

SINGLE CELL TYPE

PPP2R2B is part of cluster 51 Oligodendrocytes - Unknown function with confidenceⁱ 1
302 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
KLHL32	Kelch like family member 32	0.7599	51
STPG2	Sperm tail PG-rich repeat containing 2	0.7476	51
CNTN2	Contactin 2	0.7257	51
SIK3	SIK family kinase 3	0.7175	51
DNAJC6	DnaJ heat shock protein family (Hsp40) member C6	0.6934	51
MVB12B	Multivesicular body subunit 12B	0.6772	51
HSD17B3	Hydroxysteroid 17-beta dehydrogenase 3	0.6751	51
RTL4	Retrotransposon Gag like 4	0.6738	51
SRCIN1	SRC kinase signaling inhibitor 1	0.6712	51
KIF6	Kinesin family member 6	0.6646	51
TTLL11	Tubulin tyrosine ligase like 11	0.6637	51
OTUD7A	OTU deubiquitinase 7A	0.6578	51
MAGI2	Membrane associated guanylate kinase, WW and PDZ domain containing 2	0.6569	51
CTNNA3	Catenin alpha 3	0.6548	51
TMEM151A	Transmembrane protein 151A	0.6508	51

IMMUNE CELLS

PPP2R2B is part of cluster 1 T-cells - Unknown function with confidenceⁱ 0.99
202 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
APOBEC3H	Apolipoprotein B mRNA editing enzyme catalytic subunit 3H	1.0000	1
POGLUT2	Protein O-glucosyltransferase 2	1.0000	1
EFCAB14	EF-hand calcium binding domain 14	1.0000	1
BTN3A3	Butyrophilin subfamily 3 member A3	0.9762	1
GNL1	G protein nucleolar 1 (putative)	0.9762	1
TNFRSF18	TNF receptor superfamily member 18	0.9762	1
NLRC3	NLR family CARD domain containing 3	0.9762	1
PYHIN1	Pyrin and HIN domain family member 1	0.9762	1
CEP78	Centrosomal protein 78	0.9762	15
ZNF365	Zinc finger protein 365	0.9762	15
YWHAQ	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta	0.9762	1
FAHD2B	Fumarylacetoacetate hydrolase domain containing 2B	0.9524	1
GPR174	G protein-coupled receptor 174	0.9524	1
OBI1	ORC ubiquitin ligase 1	0.9524	1
ATAD1	ATPase family AAA domain containing 1	0.9524	15