Single cell type - PALM2AKAP2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	PALM2AKAP2
Gene descriptionⁱ Full gene name according to HGNC.	PALM2 and AKAP2 fusion
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	12

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity.	Adipocytes & Endothelial cells - Mixed function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Cell type enhanced (Alveolar cells type 1, Adipocytes, Cardiomyocytes, Endothelial cells, Granulocytes, Lymphatic endothelial cells)
Tau specificity scoreⁱ Tau specificity score is a numerical indicator of the specificity of the gene expression across cells or tissues. The value ranges from 0 and 1, where 0 indicates identical expression across all cells/tissue types, while 1 indicates expression in a single cell/tissue type.	0.54
Brain cluster specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell cluster types based on snRNA-seq data from brain samples. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. "cell type" refer to one of the 34 cluster names used to summarize the 2.5 million cells included in the brain cell representation.	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Immune cell enhanced (memory B-cell, naive B-cell)
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); neXtProt protein existence (neXtProt evidence);and a Human Protein Atlas antibody- or RNA based score (HPA evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable.	Evidence at protein level

ASSAYSⁱ

Single cell type

Single nuclei - Brain

Immune cells

Expression clustering

SINGLE CELL TYPESⁱ A summary of normalized single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types RNA single cell type specificity: Cell type enhanced (Alveolar cells type 1, Adipocytes, Cardiomyocytes, Endothelial cells, Granulocytes, Lymphatic endothelial cells)

SINGLE NUCLEI BRAINⁱ

Single nuclei cluster types

RNA brain cluster specificity: Low cell type specificity

IMMUNE CELL TYPE EXPRESSION (RNA)ⁱ Blood cell type expression overview shows RNA-seq data from three different sources: Internally generated Human Protein Atlas (HPA) data, as well as data generated by (Monaco et al) and (Schmiedel et al). Color-coding is based on blood cell type lineages, including B-cells, T-cells, NK-cells, monocytes, granulocytes and dendritic cells as well as total PBMC. To access sample data, click on tissue name or bar.
HPA datasetⁱ The resulting transcript expression values calculated as nTPM, resulting from the internal normalization pipeline for 18 immune cell types and total peripheral blood mononuclear cells (PBMC). Lineage Expression Alphabetical Immune cell type specificity: Immune cell enhanced (memory B-cell, naive B-cell)
Monaco datasetⁱ The transcript expression values (nTPM) resulting from the internal normalization pipeline are visualized for 29 blood cell types and total peripheral blood mononuclear cells (PBMC) from Monaco et al. Lineage Expression Alphabetical
Schmiedel datasetⁱ Transcript Per Million (TPM) values are visualized for 15 blood cell types from the Schmiedel et al. dataset. Lineage Expression Alphabetical

EXPRESSION CLUSTERING & CORRELATIONⁱ

SINGLE CELL TYPE

PALM2AKAP2 is part of cluster 41 Adipocytes & Endothelial cells - Mixed function with confidenceⁱ 1
325 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
SWAP70	Switching B cell complex subunit SWAP70	0.5973	73
LIMS2	LIM zinc finger domain containing 2	0.5501	32
IL1RL1	Interleukin 1 receptor like 1	0.5348	30
CYSLTR1	Cysteinyl leukotriene receptor 1	0.5336	73
AQP1	Aquaporin 1 (Colton blood group)	0.5256	41
CLIC4	Chloride intracellular channel 4	0.5231	32
EPAS1	Endothelial PAS domain protein 1	0.5157	41
LRRC32	Leucine rich repeat containing 32	0.5095	41
PKIG	CAMP-dependent protein kinase inhibitor gamma	0.5011	32
PLCG2	Phospholipase C gamma 2	0.4930	73
HEG1	Heart development protein with EGF like domains 1	0.4904	41
CCDC69	Coiled-coil domain containing 69	0.4903	77
SYNPO	Synaptopodin	0.4893	32
AIDA	Axin interactor, dorsalization associated	0.4851	32
FERMT2	FERM domain containing kindlin 2	0.4845	32

IMMUNE CELLS

PALM2AKAP2 is part of cluster 17 B-cells - Immunoglobulins with confidenceⁱ 1
129 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
E2F5	E2F transcription factor 5	1.0000	17
RASSF6	Ras association domain family member 6	1.0000	17
BTNL9	Butyrophilin like 9	1.0000	17
UCHL1	Ubiquitin C-terminal hydrolase L1	1.0000	17
GPM6A	Glycoprotein M6A	1.0000	17
SYBU	Syntabulin	1.0000	17
DNAJC5B	DnaJ heat shock protein family (Hsp40) member C5 beta	1.0000	17
BCL2L10	BCL2 like 10	1.0000	17
CHAD	Chondroadherin	1.0000	17
SOX5	SRY-box transcription factor 5	1.0000	17
COCH	Cochlin	1.0000	17
FCRL2	Fc receptor like 2	1.0000	17
SGO1	Shugoshin 1	1.0000	17
IGLL1	Immunoglobulin lambda like polypeptide 1	1.0000	17
CPNE5	Copine 5	1.0000	17