Single cell type - ANKRD55 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ANKRD55
Gene descriptionⁱ Full gene name according to HGNC.	Ankyrin repeat domain 55
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	4

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity.	Neurons - Synaptic function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Group enriched (Early spermatids, Inhibitory neurons, Late spermatids, Spermatocytes)
Tau specificity scoreⁱ Tau specificity score is a numerical indicator of the specificity of the gene expression across cells or tissues. The value ranges from 0 and 1, where 0 indicates identical expression across all cells/tissue types, while 1 indicates expression in a single cell/tissue type.	0.90
Brain cluster specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell cluster types based on snRNA-seq data from brain samples. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. "cell type" refer to one of the 34 cluster names used to summarize the 2.5 million cells included in the brain cell representation.	Group enriched (CGE interneuron, LAMP5-LHX6 and Chandelier, MGE interneuron, Eccentric medium spiny neuron, Midbrain-derived inhibitory, Oligodendrocyte precursor cell, Lower rhombic lip, Splatter, Medium spiny neuron, Committed oligodendrocyte precursor)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Group enriched (basophil, eosinophil, naive CD4 T-cell)
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); neXtProt protein existence (neXtProt evidence);and a Human Protein Atlas antibody- or RNA based score (HPA evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable.	Evidence at protein level

ASSAYSⁱ

Single cell type

Cell type enrichment

Single nuclei - Brain

Immune cells

Expression clustering

SINGLE CELL TYPESⁱ A summary of normalized single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types RNA single cell type specificity: Group enriched (Early spermatids, Inhibitory neurons, Late spermatids, Spermatocytes)

TISSUE CELL TYPEⁱ The tissue cell type section provides a cell type specificity prediction for all protein coding genes. More information on the data available can be found here and a description of the analysis here.
	Core cell typesⁱ The core cell type table displays data for a panel of eight cell types that are found in many tissues. Colored dots indicate that the selected gene has core cell type specificity in the indicated tissue. Grey dots indicate that cell type is present within that tissue, but the selected gene is not predicted to be enriched there. Category Alphabetical Enrichment Tissue specific cell typesⁱ This plot shows the enrichment score of the selected gene in all non-core cell types found within the profiled tissues, allowing comparisons between different cell types within different tissues. An enlarged symbol indicates classification of the gene as cell type enriched in the corresponding cell type.

SINGLE NUCLEI BRAINⁱ

Single nuclei cluster types

RNA brain cluster specificity: Group enriched (CGE interneuron, LAMP5-LHX6 and Chandelier, MGE interneuron, Eccentric medium spiny neuron, Midbrain-derived inhibitory, Oligodendrocyte precursor cell, Lower rhombic lip, Splatter, Medium spiny neuron, Committed oligodendrocyte precursor)

IMMUNE CELL TYPE EXPRESSION (RNA)ⁱ Blood cell type expression overview shows RNA-seq data from three different sources: Internally generated Human Protein Atlas (HPA) data, as well as data generated by (Monaco et al) and (Schmiedel et al). Color-coding is based on blood cell type lineages, including B-cells, T-cells, NK-cells, monocytes, granulocytes and dendritic cells as well as total PBMC. To access sample data, click on tissue name or bar.
HPA datasetⁱ The resulting transcript expression values calculated as nTPM, resulting from the internal normalization pipeline for 18 immune cell types and total peripheral blood mononuclear cells (PBMC). Lineage Expression Alphabetical Immune cell type specificity: Group enriched (basophil, eosinophil, naive CD4 T-cell)
Monaco datasetⁱ The transcript expression values (nTPM) resulting from the internal normalization pipeline are visualized for 29 blood cell types and total peripheral blood mononuclear cells (PBMC) from Monaco et al. Lineage Expression Alphabetical
Schmiedel datasetⁱ Transcript Per Million (TPM) values are visualized for 15 blood cell types from the Schmiedel et al. dataset. Lineage Expression Alphabetical

EXPRESSION CLUSTERING & CORRELATIONⁱ

SINGLE CELL TYPE

ANKRD55 is part of cluster 65 Neurons - Synaptic function with confidenceⁱ 1
250 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
NMBR	Neuromedin B receptor	0.6402	69
COL25A1	Collagen type XXV alpha 1 chain	0.6331	65
INSL6	Insulin like 6	0.6123	18
UNC13C	Unc-13 homolog C	0.6091	65
CRACD	Capping protein inhibiting regulator of actin dynamics	0.5975	65
NRIP3	Nuclear receptor interacting protein 3	0.5937	65
PIP5K1B	Phosphatidylinositol-4-phosphate 5-kinase type 1 beta	0.5852	65
GRIN3A	Glutamate ionotropic receptor NMDA type subunit 3A	0.5738	65
RAB3C	RAB3C, member RAS oncogene family	0.5631	65
GRIP1	Glutamate receptor interacting protein 1	0.5626	65
SLC35F4	Solute carrier family 35 member F4	0.5614	65
AMN1	Antagonist of mitotic exit network 1 homolog	0.5573	70
CDH13	Cadherin 13	0.5498	65
GRIK1	Glutamate ionotropic receptor kainate type subunit 1	0.5455	65
PTCHD4	Patched domain containing 4	0.5364	65

IMMUNE CELLS

ANKRD55 is part of cluster 36 Eosinophils - Protein ubiquitination with confidenceⁱ 0.56
179 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
NDFIP2	Nedd4 family interacting protein 2	1.0000	36
ELOB	Elongin B	0.9762	36
TM2D1	TM2 domain containing 1	0.9762	36
MED21	Mediator complex subunit 21	0.9762	36
TBRG1	Transforming growth factor beta regulator 1	0.9762	36
CDC26	Cell division cycle 26	0.9524	36
HECTD1	HECT domain E3 ubiquitin protein ligase 1	0.9524	36
CHRNB1	Cholinergic receptor nicotinic beta 1 subunit	0.9524	36
TTC7B	Tetratricopeptide repeat domain 7B	0.9524	22
TMEM65	Transmembrane protein 65	0.9524	36
MEGF6	Multiple EGF like domains 6	0.9524	22
ZNF230	Zinc finger protein 230	0.9524	36
GGPS1	Geranylgeranyl diphosphate synthase 1	0.9524	36
THYN1	Thymocyte nuclear protein 1	0.9524	36
AGO3	Argonaute RISC catalytic component 3	0.9524	36