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Diffuse-synaptic and axonal immunostaining throughout the brain with variable intensities. Generally weak-moderate but it is strong in the lateral septum, nucleus accumbens shell, ventral pallidum, BNST, globus pallidus, preoptic area, hippocmapus, substantia nigra.
In addition, a subset of neurons exhibit somatic (cytoplasmic) immunostaining, like mitral cells in the olfactory bulb or some neurons in the neocortex layer IV-V.
In addition, a subset of glial cells (possibly astrocytes, with their endfeets) is also immunoreactive throughout the brain; more studies are needed to identify their exact type.
Positive cells and structuresi
Manually selected location of the protein positivity, observed by immunofluorescence staining in mouse brain.
Soma and endfeet in astrocytes. Soma, axon and synapse in neurons.